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The weight of the tibialis anterior (TA) muscles from aged mice was decreased when compared to that from young mice, whereas its body weight was rather increased compared with young mice (Fig.?1A). Muscle glycogen content, reflecting the muscle function, was also decreased in the muscle from aged mice compared with that from young mice (Fig.?1B), suggesting that aged mice exhibit sarcopenia. As it has been shown that activation of Wnt/��-catenin signal can be seen in mSCs and their derived myogenic cells of the skeletal muscles from aged mice compared to those from young mice, we examined expression levels of a representative target http://www.selleckchem.com/products/birinapant-tl32711.html gene of Wnt/��-catenin signal (Axin2) following skeletal muscle injury of young and aged mice with cardiotoxin (Ctx) (see ��'Experimental procedures'��). As shown in Fig.?1C, expression of Axin2 was induced at higher levels during myogenic differentiation and/or muscle regeneration in the TA muscles from aged mice compared to those from young mice following Ctx-induced TA muscle injury, indicating that Wnt/��-catenin signal was activated at higher levels in the muscle tissues from aged mice than in those from young mice following muscle injury. We next examined a possible age-related alteration in myogenic differentiation and/or muscle regeneration in the TA muscles following Ctx-induced muscle injury. It was found that damaged muscles from aged mice were mainly composed of very thin myofibers ( http://www.selleck.cn/products/azd9291.html between young and aged mice, we carried http://www.selleckchem.com/products/Gemcitabine-Hydrochloride(Gemzar).html out fluorescence-activated cell sorting (FACS) analysis using satellite cell-specific antibody, SM/C-2.6 antibody. FACS dot plots showed that the relative contents of mSCs were decreased in the TA muscles from aged mice (Fig.?2A,B), whereas expression of Pax7 in mSCs from aged mice was comparable to that in mSCs from young mice (Fig.?2C). Next, we carried out comparative expression analysis of Wnt receptors (all Fzds, Lrp5 and Lrp6) in mSCs of the TA muscles from young and aged mice. Expression of Fzd1 in mSCs from aged mice exhibited significantly higher levels than that from young mice, whereas expression of any other receptor genes in mSCs from both young and aged mice was almost comparable (Fig.?2D), suggesting that high levels of Fzd1 in mSCs might be involved in activation of Wnt/��-catenin signal in myogenic cells from aged mice during myogenic differentiation. To further examine this possibility, we carried out in vitro differentiation assay using isolated mSC-derived myogenic cells from young and aged mice. Fzd1 and the typical target gene of Wnt/��-catenin signal (Axin2) were highly expressed in myogenic cells from aged mice up to day 7 after differentiation (Fig.?3A, upper panels).