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All samples were rehydrated in 1?ml distilled water for 15?min at 25��C with gentle shaking. Then, samples were plated as described above and incubated for 48?h at 37��C in microaerophilia. Cell viability obtained for each lyoprotectant was expressed as Survival Factor (SF), calculated using the following equation: Cell viability during storage was expressed as Survival Factor during t month of Storage (SFSt) and calculated as follows: To assess the maintenance of http://www.selleckchem.com/products/MG132.html the beneficial properties of selected LAB strains, the degree of surface hydrophobicity and autoaggregation and the inhibitory activity against pathogens were determined before and after freeze-drying and during storage. Dried LAB cells were rehydrated as described previously and grown in LAPTg or MRS broth for 12?h. The degree of hydrophobicity was determined by the microbial adhesion to hydrocarbons (MATH) assay modified as described by Otero et?al. (2004) and autoaggregation according to Montel Mendoza et?al. (2012). Inhibitory activity was determined by the agar-well diffusion assay using Pseudomonas aeruginosa (an indigenous RLS-related pathogen) and Listeria monocytogenes Scott A (a food-borne bacterium) as indicator strains (Pasteris et?al. 2009a). All experiments were performed in duplicate. Cell viability data were analysed by an anova-general linear model for analysis of residues to determine the effect of the variables (strain and drying medium) and the interactions http://www.selleckchem.com/products/Rapamycin.html of those effects on cell viability during the freeze-drying process. anova tests were used to quantify the effect of storage conditions (medium, temperature and time) on http://www.selleck.cn/products/Bleomycin-sulfate.html LAB viability during 18?months of storage. Significant differences between the mean values of each treatment were determined using Fisher's LSD test (95% confidence interval). Statistical analysis of the data was carried out with InfoStat 2008 (student version; National University of C��rdoba, C��rdoba, Argentina). The resistance of four selected beneficial LAB strains to the freeze-drying process was determined using nine lyoprotectants. Results showed significant differences in cell viability in all strains during the process (P?