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The Shannon diversity index computed for every host�Cmicrohabitat�Cmarker combination ranged from 3.18 to 5.34 suggesting that the fungal communities are highly diversified. It is noticeable that for each sampling site and markers, the lowest diversity and evenness indices are found in the immersed A.?marina samples and the highest indices are observed in the emerged R.?stylosa. However, no specific pattern is identifiable for the emerged A.?marina or the immersed R.?stylosa, with contradicting indications from the different markers. To compare the OTU composition of the fungal communities in each sample, a nMDS was performed based on the Bray�CCurtis dissimilarity (Fig.?2). For the four different markers, this ordination method indicates that the http://www.selleck.cn/products/carfilzomib-pr-171.html http://www.selleckchem.com/products/Metformin-hydrochloride(Glucophage).html samples collected from the same host microhabitat harbour similar communities and that communities found in the four host microhabitats are different from one another. To validate this result, we used two statistical tests: the anosim, a distribution-independent analogue of one-way anova; and the npmanova. Both tests require to establish a priori groups of data and use permutation (n?=?10?000) to assess the statistical significance of the results. Given the indication of the nMDS, the data from the same host microhabitat were grouped together (i.e. groups are ��AB-��AB-��AB; ��AH-��AH-��AH; ��RB-��RB-��RB; ��RH-��RH-��RH). anosim results indicate a strong similarity of the data within the groups (ITS1F: R?=?0.972 P? http://www.selleckchem.com/products/BIBF1120.html points out that the different environmental conditions encountered at the three sampling sites have a much smaller impact on the fungal community's composition, although the variations in the diversity indices denote a spatial patchiness (Table?1). To check the effect of low-abundant OTUs on these results, the OTUs distribution in the complete matrices (containing all OTUs) was analysed and provided similar results (Fig. S4). To evaluate the taxonomic composition of each tree-level sample, the representative reads were compared, using blastn, with two databases containing only fungal sequences and then classified using megan and the NCBI taxonomy. The taxonomic assignment at the specie level for the 25 most abundant OTUs in each sample is given in Table S1.