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The RI was defined as the ratio of the measured current amplitude to the predicted value at +60 mV (extrapolated from the linear fit of the I�CV relationship from ?90 to 0 mV). For long-term potentiation (LTP) or LTD experiments, a stable baseline was recorded for at least 5 min, followed by an additional ?25 min of recording after applying the LTP/LTD induction protocols. Synaptic responses were normalized to the averaged value of the baseline responses, and the normalized synaptic responses recorded ?20�C25 min after LTP/LTD induction were averaged and used for statistical comparison. All data are presented as the mean ��s.e.m. The non-parametric Mann�CWhitney�CWilcoxon U test was used for the statistical comparison of parameters between CT EPSCs and ML EPSCs and the Wilcoxon matched-pairs sign-ranks test was used to compare responses before and after http://en.wikipedia.org/wiki/MERTK LTP/LTD induction http://www.selleckchem.com/products/SRT1720.html or between simultaneously-recruited CT EPSCs and ML EPSCs. The statistical significance of the correlation analyses was assessed by the non-parametric Spearman's rank-order correlation test. The criterion for significance was P http://www.selleckchem.com/products/Adrucil(Fluorouracil).html staining without further sectioning. Briefly, the slices were treated with 3% H2O2 in PB for 30 min, then with phosphate-buffered saline containing 0.03% Triton X-100 (PBST). They were then incubated with 2% bovine serum albumin and 10% normal goat serum in PBST for 1 h at room temperature, followed by overnight incubation at 4��C with avidin-biotinylated horseradish peroxidase complex in PBST (1:100 dilution; Vector Laboratories, Burlingame, CA, USA). Finally, bound horseradish peroxidase was visualized using 3,3-diaminobenzidine (DAB) as the chromogen. The staining results for the biocytin-filled neurons were examined and photographed under bright-field microscopy. All of the recorded neurons were located in the VBN (Fig. 1A), as confirmed by post hoc histochemistry visualizing the injected biocytin. They had a polygonal soma, six to 10 large primary dendrites, and the bushy appearance of dendritic arborization (Fig. 1B). They all displayed Vm-dependent dual (continuous and burst) firing modes, with the watershed for mode switching at ???60 mV (Fig. 1C). Since all of these physiological and morphological properties are consistent with previous reports (Peschanski et al. 1984; Jahnsen & Llinas, 1984) and there are no GABAergic interneurons in the VBN in rats (Spacek & Lieberman, 1974), we presumed that the recordings were made from TC relay neurons.