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When compared to a larger group of epithelial and smooth muscle cells (CD11b?, CD31?), the Type II cells demonstrated significantly enhanced expression of 14 angiogenesis-associated genes (P http://en.wikipedia.org/wiki/Diglyceride 7). When the Type II cells on Day 7 after pneumonectomy were compared to nonsurgical controls, 10 genes demonstrated significantly increased expression (P http://www.selleckchem.com/products/AZD0530.html defined as a CD45?, MHC class II+, phosphine+ phenotype, demonstrated an increase in cell number after pneumonectomy; 2) the increase in cell number preceded the increase in Type I (T1��+) cells, and 3) did not appear to involve the contribution of blood-borne Type II (or Type I) cells. 4) The CD45?, MHC class II+, phosphine+ cells demonstrated the active transcription of angiogenesis-related genes both before and after pneumonectomy. Together, the data suggest the local contribution of alveolar Type II cells to http://www.selleckchem.com/products/GDC-0941.html alveolar growth. Our definition of alveolar Type II cells was based on cytologic and morphologic features; that is, cuboidal morphology and ultrastructural lamellar bodies. The cuboidal morphology produced a distinctive ��optical phenotype�� (Wilson et al., 1986) detected by flow cytometry light scatter analysis. The lamellar bodies, subcellular structures containing the lipid�Cprotein complex of the surfactant system (Ochs, 2010), were detected using the lipid-soluble fluorescent dye phosphine (Uhal and Etter, 1993; Harrison et al., 1995) and flow cytometry. The selectivity of phosphine binding to lamellar bodies has been demonstrated by confocal microscopy (Bakewell et al., 1991).