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All statistical analyses were carried out using a commercially available software program (SAS Enterprise Guide 4.1; SAS Institute, Cary, NC, USA). The Kaplan�CMeier method was used to calculate unadjusted estimates of azoospermia. Odds ratios and the corresponding 95% confidence intervals were used to measure the strength of the association. The ��2-test, Kruskal�CWallis test and t-tests (or Wilcoxon rank sum test) were used to compare parameters between groups, and multiple logistic regression analysis was carried out to assess the independent contributions of variables on the development of azoospermia. Cox's proportional hazards model analysis was used to estimate hazard ratio, and http://www.selleckchem.com/products/bay-57-1293.html logistic regression analysis was applied to discover the risk of becoming azoospermic with every 1 million decrease in sperm count. One-way analysis of variance (anova) http://www.selleck.cn/products/CAL-101.html was utilized to evaluate vitality, morphology and motility of each subgroup. A receiver operating characteristic (ROC) curve analysis was carried out to determine the threshold sperm concentration. Statistical significance was defined as a P-value http://www.selleckchem.com/products/gsk2126458.html sperm concentration from the initial concentration of 16.11?��?2.42*106/mL to 17.85?��?2.56*106/mL after 211?��?189.49?days then 19.47?��?4.84*106/mL after 287?��?319.49?days. A total of 13 men in the mild oligozoospermia subgroup achieved fatherhood before undergoing a third semen analysis. Not a single man with a mild degree of oligozoospermia developed azoospermia on their natural course, whereas six men in the subgroup showed transient worsening to the level of moderate oligozoospermia that eventually recovered back to the mild degree and even to the normal ranges. As shown in Figure?1, the Kaplan�CMeier method was used to calculate unadjusted estimates of progression to azoospermia (P?=?0.0035). The median duration to developing azoospermia in the present study group was 1193?days, with a standard error of 94.6?days. The subgroup of severe oligozoospermia had statistically smaller testicles than the other two groups (11.9 vs 14.3?mL vs 14.