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Each recipient testis was gently microinjected with 200 ��L of a solution containing 5 �� 106 cells and the testes were then reintroduced into the scrotum. In order to avoid inflammatory response, the animals received 2.2 mg/kg BW of ketoprofen (Ketojet; Uni?o Qu��mica Farmac��utica Nacional S/A, Embu-Gua?u, Brazil) and 2.5 mg/kg BW of enrofloxacin (Flotril; Ind. Quim. Farm Schering-Plough S/A, Rio de Janeiro, Brazil). For syngeneic transplantation, the recipient testes from the 4 domestic cats http://www.selleck.cn/products/ipi-145-ink1197.html were collected 3, 5, and 7 weeks after the injection of the germ cells. For xenogeneic transplantation, the recipient testes from the 7 cats were collected between 2 and 13 weeks. Transplantation was performed on all recipient cats 7 weeks postradiation. To evaluate transplantation efficiency (colonization and donor germ cell differentiation) and the presence of PKH26-GL labeled donor germ cells, several testis fragments were snap frozen in liquid nitrogen and further cryosectioned (?5 ��m in thickness). To evaluate the extension of donor germ cell colonization in isolated seminiferous tubules through http://www.selleckchem.com/products/SB-431542.html fluorescence microscopy, fresh testis fragments were also further digested with collagenase and hyaluronidase, as previously described. All data are presented as mean �� SEM and were analyzed using Student's t test. Analysis was performed using the STATISTICA 3.11 software program for Windows (StatSoft Inc, Tulsa, Oklahoma). The significance level was set at P http://www.selleckchem.com/products/byl719.html (Figure 2). Animals that received 5 mg and 5 + 5 mg of busulfan respectively (Figure 2B and C) exhibited 0.6% and 5% affected seminiferous tubules with various degrees of spermatogenesis alterations. These alterations were more pronounced in the animals that received 10 and 10 + 10 mg of busulfan (13% and 55%, respectively; Figure 2D and E). In the animals injected with 15 and 15 + 15 mg of this cytostatic drug (Figure 2F and G), 70% and 82% of the tubules exhibited prominent disturbed spermatogenesis, respectively. Among the affected seminiferous tubules evaluated using the 2 higher doses, approximately 15% and 45% were composed only of Sertoli cells. Unfortunately, 1 domestic cat treated with 15 + 15 mg and those treated with 25, 25 + 25, 35, and 35 + 35 mg of busulfan died during the experimental period, likely because of the high doses. This suggests that busulfan treatment at doses equal to or greater than 15 + 15 mg is not a feasible approach for depleting endogenous spermatogenesis in the domestic cat. The Table displays the data on the adult cats submitted to x-ray radiation.