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We tested the interactions between LIHI and LICI by comparing the effects of applying LIHI and LICI together to that of LIHI or LICI alone. The test states are shown in Table 2. Seven states were delivered in random order and repeated 10 times for each. The first three states (3A�C3C) assessed the inhibitory effect of CS100 (3B/3A) or CCS40 (3C/3A) on a test MEP of 1 mV. The TS intensity was increased in states 3D�C3G to produce a 1 mV MEP in the presence of CS100 (TS1mVCS100). The experimental design allowed us to compare the degree of LIHI in the presence of LICI (3G/3E) to LIHI alone matched for MEP amplitude (3C/3A) and TS intensity (3F/3D). We also compared the LICI with 1 mV test MEP (3B/3A), LICI with test intensity of TS1mVCS100 (3E/3D) and http://www.selleckchem.com/products/AZD0530.html LICI in the presence of LIHI (3G/3F). In this comparison, 3B/3A and 3G/3F were not matched for MEP amplitude, but 3E/3D http://www.selleckchem.com/products/GDC-0941.html and 3G/3F were matched for TS intensity. MEP amplitudes were measured peak to peak. SICI, ICF, LICI and LIHI were expressed as the ratio of the conditioned (with preceding CS) to the unconditioned (TS alone) MEP amplitudes. A ratio more than 1 indicates facilitation and less than 1 indicates inhibition. Values were expressed as mean �� standard deviation (s.d.). For Experiment 1, the effects of different test MEP amplitudes on SICI, ICF, LICI and LIHI were tested by repeated-measures analysis of variance (ANOVA) and Fischer's protected least significance difference (PLSD) post hoc test. For Experiment 2, the effects of LIHI on SICI were determined by repeated measures ANOVA by comparing SICI for TS1mV (2B/2A), SICI for a TS1mVCCS40 (2F/2E) and SICI in the presence of LIHI (2I/2H) as the repeated measures. Similarly, ICF for TS1mV (2C/2A), ICF for TS1mVCCS40 (2G/2E) and ICF in the presence of LIHI (2J/2H) were compared. For Experiment 3, the effects of LICI on LIHI were examined by repeated measures ANOVA with test conditions of LIHI for TS1mV (3C/3A), LIHI for TS1mVCS100 (3F/3D) and LIHI in the presence of LICI (3G/3E) as repeated measures. Similarly, the effects of LIHI on LICI were examined by repeated measures ANOVA with test conditions LICI for TS1mV (3B/3A), LICI for TS1mVCS100 (3E/3D) and LICI in the presence of LIHI (3G/3F) as repeated measures. Post hoc Fischer's PLSD test was used to examine which condition was different from others if ANOVA showed significant main effect. http://en.wikipedia.org/wiki/Diglyceride Bartlett's test was used to test for sphericity. The threshold for significance was set at P