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injection of sodium pentobarbital (30 mg kg?1) at PD5. A glass pipette filled with 5 ��l of drug solution was stereotaxically inserted at AP �C0.5 mm, ML +0.7 mm and DV + 2.0 mm from bregma. The CCK-1 receptor antagonist (LGM; 100 ��m), CCK-2 receptor antagonist (CI-988; 100 ��m) or vehicle (saline containing 0.1% (v/v) DMSO) was injected into the lateral ventricle using a microinjector (Model IM-108; Narishige, Tokyo, Japan; net injectant volume ? 2 ��l). Pups were then returned to their original cages. Dams were fed high-fat diets after delivery. Pup body weights were measured at PD5, PD6 and PD10. To visualize cerebral distribution of LGM following microinjections, fluorescent 4-(N,N-dimethylaminosulfonyl)-7-piperazinobenzofurazan (DBD-PZ) http://www.selleckchem.com/products/BEZ235.html linked to the carboxyl terminal of LGM (LGM/DBD-PZ; gifts from Dr Naoki Toyooka at the University of Toyama) was microinjected http://www.selleck.cn/products/gsk-j4-hcl.html as above in the wild-type pups at PD5 (n= 3). As a control, unlinked DBD-PZ (Tokyo Chemical Industry, Co., Tokyo, Japan) was microinjected in a separate group (n= 3). Brains were collected for cryostat sections 24 h after injection, and LGM labelling was visualized by the fluorescence intensity of LGM/DBD-PZ, which was subtracted from non-specific DBD-PZ background levels. Data are presented as the means with standard error. Two-way analysis of variance (ANOVA) or one-way ANOVA followed by Duncan's multiple range tests were used for statistical comparison across multiple means. The two-tailed Student's t test was used for pairwise comparisons. A 95% confidence level was considered to indicate statistical significance. CCK1R�C/�C mice had normal body weights at PD3 (n.s.; Fig. 1Aa), but they grew faster during the subsequent pre-weanling stage. http://www.selleckchem.com/products/byl719.html Consequently, CCK1R�C/�C mice were significantly heavier than wild-type mice at PD5 (P