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Relative quantification of gene expression with real-time PCR data was calculated relative to ��-actin. Relative quantitative measures the relative change in mRNA expression levels. It determines the changes in steady state mRNA levels of a gene across multiple samples and expresses it relative to the other levels of RNA (Wong and Medrano 2005). Data from individual experiments are expressed as the mean?��?SD deviation. The data were analysed by one-way anova using spss 12.0.1 program (SPSS Inc., Chicago, IL, USA), and the differences between the means assessed http://www.selleckchem.com/products/Everolimus(RAD001).html using Duncan's multiple range test. Statistical significance was considered at P-value? http://www.selleck.cn/products/PD-98059.html group (P? http://www.selleckchem.com/products/Rapamycin.html group than in the HDC group (P?