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FNA was directed to the kidney in 7 cases; the gluteus in 3 cases; the back in 2 cases; and the abdominal cavity, parotid gland, mediastinum, infraumbilical, supraclavicular, pancreas, retropharynx, and temporal areas in 1 case each (Table 1). All smears were stained according to the May-Grunwald-Giemsa method and before 2000 by immunocytochemistry. Genetic and/or molecular analysis was performed on 9 samples using cytologic material. For molecular studies, at the time of FNA, aspirated material was rinsed into Vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) containing 3 mL of ethylenediamine tetra-acetic acid in Hank balanced salt solution. These tubes were immediately transported http://www.selleckchem.com/products/blz945.html to the genetics laboratory for a cell count. When a minimum of 5 million cells were available, the cell suspension was equally divided: 1 portion for karyotyping and the other for reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Priority was given to RT-PCR analysis when http://www.selleck.cn/products/BafilomycinA1.html using phenol-chloroform and light-phase lock-gel procedures. For the analysis of genomic DNA, primer couples located in introns and amplifying the 9 exons of SMARCB1 were designed. PCR products were purified and sequenced bidirectionally using the Big dye terminator V3.1 kit (Applied Biosystems, Foster City Calif) on automatic 3130 XL sequencers. Sequences were analyzed by SeqScape software PCR. The sequences of the primers were retrieved from the Genome Database (mirror site available at: http://gdb.infobiogen.fr/ accessed October 7, 2010), the Whitehead Institute/Massachusetts http://www.selleckchem.com/products/hydroxychloroquine-sulfate.html Institute of Technology genome center (available at: http://www-genome.wi.mit.edu/ accessed October 7, 2010), the Human Gene Map (available at: http://www.ncbi.nlm.nih.gov/SCIENCE96/ accessed October 7, 2010), the Cooperative Human Linkage Center (available at: http://gai.nci.nih.gov/CHLC/ accessed October 7, 2010), the Stanford Human Genome Center (available at: http://www-shgc.stanford.edu accessed October 7, 2010), and the Sanger Center (available at: http://www.sanger.ac.uk/ accessed October 7, 2010).11 In the group of 12 primary tumors, core needle biopsies (18-gauge) for histologic study were performed in 9 cases immediately after FNA sampling.