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69%, 45.78%, and 53.37%, respectively (P http://www.selleckchem.com/products/Dasatinib.html found to be the major component of EAE chromatogram. In this study, our data show that EAE and CE could inhibit the proliferation of all five human cancer cell lines in a dose- and time-dependent manner at the concentration range of 50�C200 ��g/mL (Figs. 2, 3, and 4). In comparison with EAE and CE, less inhibitory effect was observed with PEE and NBE. The five tumor cell lines showed differential sensitivities to EAE and CE with SMMC-7721 cells being the most sensitive to EAE and CE treatment. The growth inhibitory effect of EAE and CE on SMMC-7721 cells was enhanced with the increase of drug concentrations. The highest growth http://www.selleckchem.com/screening/epigenetics-compound-library.html inhibition rates following treatments with EAE and CE, each used at the concentration of 200 ��g/mL for 72 hr, were 80.91% and 51.76%, respectively (Fig. 4). Of note, the antiproliferative effect of EAE was strongest among the four tested extracts at all observed time points. These results indicate that the active anticancer component(s) present in Euphorbia https://en.wikipedia.org/wiki/Evodiamine helioscopia L may be enriched in the EAE fraction. The data of cell cycle show that EAE mainly arrested cells in G-1 phase in a dose- and time-dependent manner and reduced the percentage of cells in S-phase. Interestingly, the percentages of cells in G2/M-phase increased at 24 and 48 hr but decreased at 72 hr, as compared with controls. A remarkably high subdiploid peak was also observed in the treatment group (Fig. 5), suggesting that EAE-induced antiproliferation may be related to apoptosis. However, the sub-G1 percentages of 150 ��g/mL treatments are smaller than those of 100 ��g/mL treatments at 24 and 48 hr (Table 1). Moreover, the similar situation existed in early apoptosis whereby the proportion of early apoptotic cells treated with 150 ��g/mL at 48 hr is smaller than that of the same concentration used at 24 hr (Fig. 6). This may be due to the reason that herbal extract is a complex mixture which is composed of many different pharmacologically active components. Interactions between different active ingredients may lead to such results.