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18, 2.52 and 3.41 corresponded to 3, 4 and 5 or more copies, whereas CNs of less than 1.75 and 1.34 corresponded to mono- and biallelic deletion. Total RNA was available in 21 http://www.selleck.cn/products/Methazolastone.html cases and purified using the RNeasy? Total RNA Isolation Kit (Qiagen, Valencia, CA). Preparation of DNA single-stranded sense target, hybridization to GeneChip? Gene 1.0 ST arrays (Affymetrix, Santa Clara, CA) and scanning of the arrays (7G Scanner, Affymetrix) were performed according to the manufacturer's protocols. Log2-transformed expression values were extracted from CEL files and normalized using NetAffx Transcript Cluster Annotations, Release 31 and robust multiarray average (RMA) procedure in Expression Console software (Affymetrix). The expression values of transcript cluster IDs specific for loci representing naturally occurring read-through transcriptions were summarized as the median value for each sample. Supervised analyses were performed using Significant Analysis of Microarrays software, version 4.0 [16] as http://www.selleckchem.com/products/Nutlin-3.html previously described [17]. The functional annotation analysis on the selected lists was performed by means of NetAffx (https://www.affymetrix.com/analysis/netaffx/) and the Database for Annotation, Visualization and Integrated Discovery (DAVID) 6.7 (U.S. National Institutes of Health at http://david.abcc.ncifcrf.gov/) tools. Only Gene Ontology Biological Process and Molecular Function terms were selected as annotation categories and high classification stringency was set for the analysis, using the DAVID Functional Annotation Clustering option. The Annotation Clusters with an Enrichment Score (ES) >1.3 were considered http://www.selleckchem.com/products/ABT-737.html significant. To evaluate whether gene dosage effects could be identified for specific genes, the relationship between relative transcript expression levels and the inferred CNs of the corresponding locus (according to NCBI36/hg18) was measured by means of Kendall's �� correlation test (P-value