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1. Significance of variance components was obtained by nonparametric procedures using 1000 random permutations. Morphological examination revealed significant phenotypic differences among plants growing in different regions and habitats. In particular, plants in aquatic habitats exhibited significantly larger leaves, longer internodes, http://www.selleckchem.com/products/Everolimus(RAD001).html and larger stem pith cavity diameter than those in terrestrial habitats (Fig.?1). The anova results indicated that both regional and local habitat factors had significant effects on morphological traits (P? http://www.selleck.cn/products/PD-98059.html DNA methylation patterns of 90 individuals from six field populations were revealed by MSAP. A total of 1000 markers were generated with nine selective primer combinations and 274 bands were polymorphic (27.4%). Different methylation states were http://www.selleckchem.com/products/Rapamycin.html observed within and among populations. The relations among individuals were shown by a PCO plot based on pairwise Dice distances (Fig.?2), where the first and second axes explained 33.98 and 20.84% of the variation, respectively. The individuals examined were grouped into three major clusters corresponding to three source field sites: Kunming, Nanning, and Zhuji. Furthermore, individuals from aquatic and terrestrial habitats tended to form distinct subgroups within each local group (Fig.?2). amova showed that a large proportion of epigenetic variation (78.7%, P?