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Nitrocellulose (0.22-��m pore size; Osmonics Inc, Westborough, Massachusetts), the polyclonal anti-histone (H2B) antibody (directed against the C-terminal of somatic H2B and present in nonsomatic variants TSH2B and H2BFWT; Millipore, Billerica, Massachusetts), the PageRuler prestained protein ladder (Fermentas Canada, Burlington, Canada), goat anti-rabbit IgG conjugated to horseradish peroxidase (Cedarlane), an enhanced luminescence kit (Lumi-Light; Roche Molecular Biochemicals, Laval, Canada), and radiographic Fuji films were used for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Fetal cord blood was collected at the birthing center of the Royal Victoria Hospital (Montr��al, Canada) after informed consent was obtained from the patients. The ethics board of the McGill University Health Centre approved this study. http://www.selleckchem.com/products/z-vad-fmk.html Blood samples were centrifuged and supernatants were pooled and frozen until use. Fetal cord serum ultrafiltrates (FCSu) were prepared with YM3 membranes (exclusion limit of 3 kd; Amicon, Oakville, Canada) (de Lamirande and Gagnon, 1995; O'Flaherty et al, http://www.selleckchem.com/products/ly2157299.html 2006a). Healthy volunteers selected for their excellent semen quality signed an informed consent as requested by the ethics board of the McGill University Health Centre; their semen samples were normal (World Health Organization [WHO], 1999), and were allowed to liquefy for 30 minutes at 37��C. Then, spermatozoa were purified by Percoll density gradient centrifugation (95%�C65%�C40%�C20% layers, made isotonic with HEPES-balanced saline; de Lamirande and Gagnon, 1995, 1998; O'Flaherty et al, 2006a). Spermatozoa in the 95% Percoll layer and those that penetrated the 65% Percoll layer (the 65%�C95% interface) were pooled and diluted to 200 �� 106 cells/mL with 95% Percoll. These preparations are devoid of immature http://www.selleck.cn/products/XL184.html and abnormal cells and of white blood cells (de Lamirande and Gagnon, 1992). Progressive sperm motility was