The Controversy Over Callous Megestrol Acetate-Procedures

Although all young and old cells at the scratch margins showed intense increases in dihydroethidium oxidation immediately after scratching, the old keratinocytes grown at 21% oxygen demonstrated a greater decrease in the DHE oxidation following scratching and migrated the fastest. These results show that old and young keratinocytes respond to oxygen tension differently and support the hypothesis that keratinocyte migration is affected by the capacity to remove ROS. ""Background.? Intermittent blood-glucose fluctuation (BGF) can accelerate diabetes and its complications, but it is still unknown whether BGF can damage the skin of patients with diabetes. Aim.? To investigate the effects of repetitive fluctuation in blood glucose concentration on the biophysical properties, structure and antioxidant capacity of diabetic mouse skin. Methods.? Mice with alloxan-induced diabetes were injected with glucose three times daily to induce repetitive BGF. Six weeks later, skin elasticity and skin hydration were measured to evaluate skin water-holding capacity and contraction function. The histological changes of skin were also studied, using haematoxylin and eosin. In addition, we compared levels of blood sugar and advanced glycation end products (AGEs), the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA) and hydroxyproline in the skin of normal mice and diabetic mice with or without BGF. Results.? The diabetic mice with BGF displayed an 18.6% increase in blood sugar level, an 11.3% increase in AGEs content and a 13.1% increase in MDA content compared with the diabetic mice (P? skin lesions in diabetic mice. Conclusions.? Repetitive fluctuation in blood-glucose concentration produced a marked detrimental effect on skin structure and function in diabetic mice. The deleterious effects of BGF on skin appeared to due at least partly to the oxidative stress. ""Please cite this paper as: Sulforaphane but not ascorbigen, indole-3-carbinole, and ascorbic acid activates the transcription factor Nrf2 and induces phase-2 and antioxidant enzymes in human keratinocytes in culture. Experimental Dermatology 2010; 19: 137�C144. Abstract:? Nrf2 is a basic leucine zipper transcriptional activator essential for the coordinated transcriptional induction of phase-2 and antioxidant enzymes. Brassica vegetables contain phytochemicals including glucoraphanin, the precursor of sulforaphane (SFN) and glucobrassicin, the precursor of indole-3-carbinole (I3C) and ascorbigen (ABG). The degradation products SFN, I3C and ABG may be capable of inducing cytoprotective genes in skin. In this study, we tested the potency of SFN, ABG and I3C in affecting Nrf2-dependent gene expression in human keratinocytes in culture.