Rumoured Media Hype On The BML-190

fetida being both MUG-negative and MUG-like negative. Representative examples of propidium iodide stained DNA profiles of earthworm coelomocytes and human U937 cell line or CRBC as standards are illustrated in Figure 5. The fluorescence intensity of the G0/G1 peaks increased in order D. veneta???E. andrei? that is, fluorophores and DNA amounts and from well-defined specimens of E. fetida cultured in Lille University. Phylogenetic tree based on mitochondrial COI gene shows three distinct clades corresponding with D. veneta, E. fetida, and E. andrei. All individuals of E. andrei were MUG-positive (Mp), and all D. veneta worms were MUG-negative (Mn). Most specimens of E. fetida worms were MUG-negative except 2 out 16 worms from Kluczbork (about 13%) and 7 out of 18 worms (39%) from Lille, which exhibited the presence of the MUG-like fluorophore (MLp). The presence of MLp does not correspond with two distinct subclades of E. fetida clade (Fig. 6). The DNA barcoding approach proved that the dense colony of relatively small composting worms from the wastewater treatment plant consisted of the mixture of three distinct species, that is, E. andrei, E. fetida, and D. veneta. After species separation, body weight gain was significantly faster in D. veneta than in the two Eisenia species. Coelomocyte-containing coelomic fluid, non-invasively retrievable from earthworm coelom by a mild electrostimulation, was analyzed by spectrofluorimetry and flow cytometry. Both techniques brought information supporting morphological recognition of syntopic specimens of D. veneta and Eisenia spp. Spectrofluorimetric spectra of coelomocyte lysates of D. veneta never exhibit features characteristic for MUG or MUG-like fluorophores; riboflavin content was much lower in D. veneta than that in Eisenia spp., while the peak of fluorescence derived from X-fluorophore was much more distinct in D. veneta than those observed in Eisenia spp. Perhaps the low riboflavin content was responsible for a lower intensity of autofluorescence of D. veneta than Eisenia spp. coelomocytes evidenced by flow cytometry. Propidium iodide staining revealed lower content of nuclear DNA of coelomocytes in D. veneta than that in Eisenia spp. In conclusion, these coelomocyte-derived features are very helpful in distinction of D. veneta from Eisenia spp., which is especially important in a case of juvenile specimens, but do not allow definitive distinction between E. andrei and E. fetida. In the latter case, the most promising were fluorescence spectra present in E.