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All groups were kept under constant observation to detect any changes in behavior or changes in water and food intake. The animals were divided into five groups: group A was the non-treated infected control, group B was the positive control (treated with 1?mg/kg of clotrimazole IG), groups C and D were the infected groups treated with 2.5 and 5?mg/kg of acetone extract of B. https://en.wikipedia.org/wiki/Oxyclozanide hartwegii or Z. caribaeum, respectively, and group E consisted of non-infected animals treated with 10?mg/kg of extract to determine the potential toxic effects of extracts. After treatment, the mice were anesthetized with 60?mg/kg of intraperitoneal sodium pentobarbital and sacrificed by cardiac puncture to collect 2�C3?ml of blood. Serum from non-heparinized blood was carefully collected http://www.selleckchem.com/products/Decitabine.html for a liver profile test. The mice were euthanized according to the official Mexican standard for the humane killing of animals (NOM-033, 1995). All animals were euthanized, and the vital organs were macroscopically analyzed. Representative fragments of the kidney, lungs and liver (to observe the toxic effects of extracts) from three mice were fixed in a 10% solution of formalin and enclosed in paraffin. Paraffin blocks were prepared after completing tissue processing in different grades of alcohol and xylenes. Sections (5?��m) were prepared from paraffin blocks using a microtome, and the sections were stained with hematoxylin�Ceosin (H&E). A Salmonella mutagenicity test using the incorporation method was performed as previously described ( Maron and Ames, 1983), using the Salmonella typhimurium strains TA98, TA100 and TA102 and the S9 fraction obtained from the liver of a male Wistar rat induced with Aroclor 1254 (New England Nuclear, North Haven, Conn) as the metabolic activation system. S. typhimurium strains were cultured for 16?h at 37?��C in a shaking water bath set at 90?rpm. In a sterile tube containing 2?ml of soft agar (at 45?��C), the bacterial cultures (100?��L) were exposed to final extract concentrations of 0.78, 1.56, 3.12, 6.25, 12.5, 25.0, 50.0, 100 and 200.0?��g/ml, or to 10?��l http://www.selleckchem.com/screening/gpcr-library.html of DMSO as a negative control. Subsequently, the suspension mixture was placed in a Petri dish containing Vogel�CBonner minimal medium. After incubation for 48?h at 37?��C, the revertant colonies (His+) were quantified. The mutagenesis positive controls used for each strain were picrolonic acid, methyl-N-nitro-N-nitrosoguanidine, mitomycin C, 2-amino-anthracene and cyclophosphamide, as previously described ( Maron and Ames, 1983). GraphPad Prism 5.0 (GraphPad Software, Inc.) was used for statistical analysis. The average lesion score on the last day of macroscopic observation was analyzed using one-way ANOVA nonparametric test and Dunnett post hoc analysis. The differences of the means of the groups were compared with the non-treated group and were considered significant when p?